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Figure 4 | Genome Biology

Figure 4

From: A functional map of NFκB signaling identifies novel modulators and multiple system controls

Figure 4

Contextual organization of the NFκB positive modulators. Organization was determined on the basis of follow-up screens with a series of dominant-negative mutants (TRAF2, MAP3K7, IKBKB, and IKBKG). If activation of the NFκB reporter was blocked by the dominant-negative mutant (≥ 70% average reduction), the positive modulator was considered upstream of the dominant-negative mutant. In the boxes upstream of TRAF2 and MAP3K7, green text identifies genes that were inhibited by both the TRAF2 and MAP3K7 dominant-negative mutants. In the boxes upstream of MAP3K7 and the IKK complex, red text identifies genes that were inhibited only by the IKBKB mutant. Genes identified as IKBKB specific showed ≥ 70% inhibition by the IKBKB dominant-negative mutant and <70% inhibition by the IKBKG dominant-negative mutant. In addition, IKBKB-specific genes were required to show a fivefold greater reduction in NFκB activation by the IKBKB dominant-negative mutant as compared with the IKBKG mutant. Those genes that were inhibited by both the IKBKB and IKBKG dominant-negative mutants were shown in black text. Genes that were not significantly inhibited by any of the dominant-negative mutants are listed in the lower right-hand box and were presumed to act downstream in the pathway or through an independent branch.

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