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Table 2 Comparison of microarray and real-time PCR performance in detection of pathogen genera (HRV, pneumovirus)

From: Optimization and clinical validation of a pathogen detection microarray

Patient ID Array WKL P value PDA genus diagnosis PCR diagnosis PCR Ct value Virus copy no.
111 35915    ND ND   
122 35887 20.87 2.47 × 10-29 Pneumovirus Pneumovirus 24.8 5.0 × 104
133 71180 22.33 6.93 × 10-62 Pneumovirus Pneumovirus 25.1 4.0 × 104
165 66691 16.95 3.49 × 10-4 Pneumovirus Pneumovirus 27.9 3.9 × 103
185* 66696    ND ND   
254 70935 25.02 2.87 × 10-39 Pneumovirus Pneumovirus 22 5.4 × 105
261* 66697    ND ND   
283* 63781 23.99 2.28 × 10-25 Pneumovirus HRV 28.3 6.1 × 104
   14.07 4.66 × 10-11 HRV    
312* 66701    ND Pneumovirus 33.7 44
321* 71006    ND Pneumovirus 31.1 340
324* 35259 20.61 3.55 × 10-94 Pneumovirus Pneumovirus 21.4 3.0 × 106
331* 66698    ND HRV 31.7 3.6 × 103
337 71192 21.73 3.49 × 10-14 Pneumovirus Pneumovirus 26.2 1.1 × 105
   8.3 1.92 × 10-4 HRV HRV 29.1 3.1 × 104
355 35662 18.00 2.97 × 10-40 Pneumovirus Pneumovirus 20.3 6.7 × 106
368* 66702    ND ND   
374 66695    ND Pneumovirus 34.1 500
378 70933 13.82 7.77 × 10-17 Pneumovirus Pneumovirus 23.9 5.4 × 105
393* 71189 25.41 1.15 × 10-18 HRV HRV 30.2 2.1 × 105
412 35890 19.66 2.42 × 10-49 Pneumovirus Pneumovirus 23.5 6.9 × 105
414 71025 49.91 1.18 × 10-65 Pneumovirus Pneumovirus 22.3 3.9 × 105
      HRV 33 2.6 × 103
461 66699    ND ND   
478 71027    ND Pneumovirus 34.8 18
483* 36053 12.17 1.47 × 10-12 Pneumovirus Pneumovirus 24.8 2.9 × 105
554 70997 78.55 4.59 × 10-120 HRV HRV 23.5 1.5 × 106
573 66700 38.09 6.26 × 10-22 HRV HRV 22.2 3.6 × 106
639* 71182 9.23 7.91 × 10-6 HRV ND   
699 71007    ND ND   
769 73067 24.62 3.70 × 10-52 Pneumovirus Pneumovirus 25.7 2.5 × 104
818 70927 10.40 1.63 × 10-8 HRV HRV 34.2 1.2 × 103
832 73068 13.52 4.54 × 10-6 Pneumovirus Pneumovirus 28.2 3.1 × 103
   40.43 1.73 × 10-36 Pneumovirus Pneumovirus 23.8 1.2 × 105
841 73070 22.11 6.80 × 10-50 Pneumovirus Pneumovirus 20.9 4.5 × 106
       35.4 8
      HRV 29.2 3.3 × 104
853* 66690    ND ND   
859 71188 72.17 1.42 × 10-128 HRV HRV 24.5 2.8 × 106
892* 68359 12.43 5.77 × 10-5 HRV Pneumovirus 34 27
      HRV 32.3 4.2 × 103
913 71028 40.67 1.60 × 10-50 Pneumovirus Pneumovirus 19.1 4.7 × 106
924* 66703 12.79 2.56 × 10-6 Pneumovirus Pneumovirus 31.5 250
      Pneumovirus 33.7 630
  1. *Hospitalized patients. RSV A patient samples. ND, none detected.