Table 4 Comparison of TraSH and GSMN-TB predictions of gene essentiality with experimentally determined phenotype

AftA

E

E

C. glutamicum

Slow growth

[60]

AroK

E

E

M. tuberculosis

Essential

[79]

Ask

E

E

M. smegmatis

Auxotroph

[80]

CysH

E

E

M. smegmatis

Auxotroph

[81]

GlnA1

E

E

M. tuberculosis

Essential

[82]

GlnA3

NE

NE

M. tuberculosis

Nonessential

[82]

GlnA4

NE

NE

M. tuberculosis

Nonessential

[82]

hemZ

E

E

M. tuberculosis

Essential

[83]

InhA

E

NE

M. smegmatis

Ts lethal

[31]

ino1

NE

NE

M. tuberculosis

Auxotroph

[84]

KasA

NE

E

M. smegmatis

Essential

[85]

LeuD

E

E

M. tuberculosis

Auxotroph

[86]

LysA

E

E

M. tuberculosis

Auxotroph

[87]

manA

E

E

M. smegmatis

Hyperseptation and loss of viability

[88]

mshB

NE

NE

M. tuberculosis

Grows poorly

[89]

mshC

NE

E

M. tuberculosis

Essential

[90]

murD

E

E

M. tuberculosis

Nonessential

[82]

murI

E

NE

M. tuberculosis

Nonessential

[82]

Ndh

NE

NE

M. smegmatis

Ts lethal

[91]

NrdF2

E

E

M. tuberculosis

Essential

[92]

OtsA

E

E

M. tuberculosis

Slow growth

[93]

OtsB2

NE

E

M. tuberculosis

Essential

[93]

panCD

E

NE

M. tuberculosis

Auxotroph

[94]

panCD

E

E

M. tuberculosis

Auxotroph

[94]

PimA

E

NE

M. smegmatis

Essential

[95]

PurC

E

E

M. tuberculosis

Auxotroph

[96]

Purl

E

E

M. tuberculosis

Auxotroph

[96]

RmlB

NE

E

M. smegmatis

Essential

[97]

TreS

NE

NE

M. tuberculosis

Nonessential

[93]