Monitoring of gene knockouts: genome-wide profiling of conditionally essential genes

Table 3 List of genes identified by MGK as important for survival upon chloramphenicol treatment

Phenotype verification of mutants	Genes
Not tested	pstS (10.5), tolA/B (7.2), ptsI (4.9), acrB (4.2), tolA/R (4.2), sufB (3.4)
Individually tested mutants	hns (12.7), dgkA (11.5), rnhA (10.2), apaH (10.0), rluD (8.6), ahpC (8.3), ompA (8.1), pstC (7.7), rfaE (7.7), arcA (6.8), yjjY (6.7), oxyR (6.2), gor (5.5), rfaF (5.0), sufD (5.0), lpp (4.9), prc (4.7), ybeX (4.6), fpr (4.6), acrA (4.5), tolQ (4.5), arcB (4.0), phoP (3.9), clpA (3.7), ydhD (3.6), mdh (3.5), yqiC (3.5), miaA (3.4), ptsH (3.4)
Individually tested mutants exhibiting a fivefold or greater killing with 18-hour exposure to chloramphenicol	dgkA (11.5), apaH (10.0), ahpC (8.3), ompA (8.1), arcA (6.8), yjjY (6.7), lpp (4.9), prc (4.7), ybeX (4.6), tolQ (4.5), arcB (4.0), clpA (3.7), mdh (3.5)

Values in parentheses are intensity ratios (normalized reference intensity value/normalized selection intensity value), and are the average of two independent, inversely labeled experiments. In the case of tolA/B and tolA/R, the origin of signal intensity could not be distinguished between two neighboring genes. MGK, Monitoring of Gene Knockouts.

ISSN: 1474-760X