Table 1 Differential expression data based on array experiments and Q-PCR of endomesoderm marker genes
EM markers | Expression | Regulation | P value | Q-PCR ratio ± error |
|---|---|---|---|---|
Brachyury 537REA_5B8 | EM | Li 1.69 Zn 0.42 | 1.08 × e-06 3.89 × e-03 | 1.64 ± 0.21 0.33 ± 0.10 |
Blimp1 537REA_16B13 | E | Li 1.33 Zn 0.42 | 3.98 × e-02 2.58 × e-03 | 1.78 ± 0.09 0.53 ± 0.04 |
CoA-reductase 536REAsu2_4L15 | EM | Li 0.40 Zn 1.31 | 5.68 × e-08 1.20 × e-03 | 0.44 ± 0.03 1.71 ± 0.50 |
Delta 536REAsu4_17C2 | SMC | Li 1.79 Zn NA | 1.64 × e-01 NA | 4.86 ± 1.05 0.95 ± 0.14 |
Dlx 537REA_9O11 | EM-Oect | Li 0.09 Zn NA | 2.60 × e-05 NA | 0.03 ± 0.00 0.58 ± 0.26 |
Endo16 536REAsu2_5N1 | E | Li 1.20 Zn 0.46 | 3.00 × e-01 2.05 × e-04 | 1.76 ± 0.34 1.70 ± 0.12a |
Eve 537REA_2G8 | E | Li 1.33 Zn nd | 4.89 × e-03 nd | 0.93 ± 0.01a 1.73 ± 0.10 |
FoxA 537REA_10P13 | EM+Oect | Li 1.37 Zn na | 1.37 × e-06 na | 3.18 ± 0.12 1.69 ± 0.43 |
GataE 537REA_3C9 | E | Li 1.55 Zn 0.48 | 2.77 × e-03 1.71 × e-03 | 3.06 ± 0.92 0.23 ± 0.04 |
Hex SpSMBLAS_124N22 | M | Li 1.54 Zn nd | 3.99 × e-01 nd | 3.22 ± 0.53 0.33 ± 0.03 |
Hox11/13b 537REA_12K1 | E | Li 1.69 Zn na | 3.44 × e-09 na | na na |
KRL PMC_BG781437 | EM | Li Zn |  | 1.90 ± 0.03 5.95 ± 0.66 |
Lox 536REAsu4_13M18 | E | Li ne Zn 0.36 | ne 5.17 × e-03 | ne 0.04 ± 0.01 |
Notch RUDIREA_30E15 | E | Li 1.27 Zn na | 4.84 × e-01 na | 1.81 ± 0.18 13.5 ± 3.41 |
P19 537REA_15K13 | PMC | Li 1.07 Zn 1.58 | 7.82 × e-01 3.43 × e-03 | 1.66 ± 0.33 2.14 ± 0.22 |
PMAR1 | PMC | Li na Zn na | na na | 1.77 ± 0.29 0.50 ± 0.02 |
Prox RUDIREA_15N17 | M | Li 0.57 Zn na | 2.40 × e-04 na | 0.81 ± 0.16 1.46 ± 0.27 |
Six3 RUDIREA_40B23 | Apical, later +EM | Li 0.72 Zn 0.10 | 7.83 × e-02 6.46 × e-03 | 0.39 ± 0.04 1.15 ± 0.15a |
SM50 RUDIREA_5L2 | PMC | Li 0.57 Zn 1.55 | 1.09 × e-05 1.20 × e-04 | 1.07 ± 0.19a 18.12 ± 2.93 |
SMAD2 RUDIREA_24O7 | PMC | Li 0.50 Zn NA | 2.49 × e-01 NA | 0.52 ± 0.09 1.20 ± 0.32 |
Snail RUDIREA_13L18 | EM | Li 0.99 Zn 0.26 | 9.72 × e-01 9.05 × e-05 | 0.24 ± 0.01 0.09 ± 0.01 |
Sox4 RUDIREA_2H10 | EM, later +apical | Li 1.15 Zn nd | 4.23 × e-01 nd | 1.67 ± 0.21 1.05 ± 0.08 |
SuH 621REA_14C17 | EM | Li 0.24 Zn NA | 3.75 × e-02 NA | 4.24 ± 0.95a 4.61 ± 1.50 |
T-Brain 621Rea_6N24 | M | Li 0.82 Zn na | 6.93 × e-01 na | 1.40 ± 0.31a 2.18 ± 0.12 |
Tbx6 RUDIREA_29D1 | M | Li 0.64 Zn 0.74 | 6.71 × e-05 1.78 × e-05 | 0.33 ± 0.15 0.16 ± 0.04 |
Unknown 536REAsu4_13G12 | EM | Li 2.23 Zn 4.05 | 2.69 × e-07 5.52 × e-10 | 4,68 ± 0.72 11,01 ± 0,27 |
Wnt3 RUDIREA_28M14 | EM | Li 0.89 Zn NA | 7.86 × e-01 NA | 2.29 ± 0.97a 0.09 ± 0.03 |
Wnt5 RUDIREA_16P23 | EM | Li 1.25 Zn NA | 1.97 × e-01 NA | 6.31 ± 2.31 1.08 ± 0.08 |
Wnt8 537REA_10K11 | EM | Li 1.35 Zn NA | 4.19 × e-03 NA | 2.49 ± 0.07 11.36 ± 1.13 |