Figure 10

cis-Decoder analysis of the Drosophila HLHmβ 5' upstream cis-regulatory region. cis-Decoder analysis of the Drosophila HLHmβ upstream region identifies neural enhancer sequences. (a) An EvoPrint of the 869 bp Drosophila HLHmβ cis-regulatory region [54] was generated using the following genomes: D. melanogaster (reference sequence), D. simulans, D. sechellia, D. yakuba, D. erecta, D. ananassae, D. persimilis, D. pseudoobscura, D. virilis, D. mojavensis and D. grimshawi. Uppercase nucleotide sequences are conserved in all of the above genomes. (b) cis-Decoder tag analysis of the HLHmβ enhancer CSBs. CSBs (6 bp or greater) were extracted from the EvoPrint shown in (a) and aligned with Drosophila cDTs from neural and mesodermal libraries. Designations adjacent to the aligned cDTs include number of perfect matches to neural (n) and mesodermal (m) enhancers analyzed in this study. (c) cDT-cataloger analysis of the aligning cDTs reveal that the HLHmβ enhancer contains elements shared with 26 other neural enhancer CSBs and one mesodermal CSB.