Top 20 central nodes representing perturbed neighborhoods, identified by Cytoscape. Green indicates downregulation, and red indicates upregulation of the gene/node in aging (6 days after L4) versus young (1 day after L4) glp-1 nematodes; darker shades indicate greater alteration. Blue borders indicate genes that were significantly different in expression individually in aging compared with young adults (P < 0.05). Nodes that are grouped into the gray cluster do not fall into a common Gene Ontology. Additional data file 4 (parts A and B) provides additional information about each of the nodes (genes) and the associated neighborhoods.