Gene expression and phenotypes of ash2 mutants. (a) Engrailed (En) expression (dotted line) in wt and ash2 mutant wing discs (top panels). Reduction of En levels (white arrows) in ash2I1 homozygous cells (bottom panels). (b) Reduction of Vg levels (white arrows) in ash2I1 homozygous cells. (c) Wing phenotype of ash2112411 homozygotes (central picture) is rescued to wt (top picture) by expression of ash2 in ash2112411 homozygotes (bottom picture). (d) Dorsal bristles of the wing margin in ash2112411 homozygotes are partially ventralized (compare central panels with upper panels). Dorsal bristles from ash2112411 homozygotes are wt if ash2 is ectopically expressed with MS-1096-Gal4 driver (bottom panels). (e) Increased cell death in ash2I1 homozygous wing discs in comparison to wt and ash2112411 homozygous wing discs as seen by TUNEL staining (red). (f) Apoptosis in ash2I1 homozygous cells. Apoptotic cells are identified by staining with anti-cleaved Caspase 3. In (a,b,f), wt cells are bright green, heterozygous cells are light green and ash2I1 homozygous cells are black. In (e,f), dotted lines outline the discs. In all wing discs, anterior is left and dorsal up.