Whole genome expression analysis of ash2 and ash1 mutants. (a) GO annotation of genes that were upregulated (black bar) or downregulated (grey bar) in each allele (see text for details). The length of the bar indicates the fold change. N indicates that no genes were found with a given term in this set. O indicates over 10 times. An asterisk indicates FDR adjusted p value < 0.01 and a plus sign indicates <0.05 (FDR adjusted p values for each GO annotation are detailed in Additional data file 21); p.f., pattern formation. (b) Expression maps including all the genes belonging to GO terms 'Wing disc development', 'Anterior/posterior pattern formation, imaginal disc' and 'Dorsal/ventral pattern formation, imaginal disc'. All the genes from the array having these GO terms are represented irrespective of log2ratios or p values. Red indicates upregulation and green downregulation (see scale bar). (c) Semi-quantitative RT-PCR and microarray results for six genes. The height of the bar is proportional to the log2 ratio observed for each gene in the microarray or RT-PCR experiments in all three alleles. The expression level of all genes was statistically significant across microarray replicate experiments in all three mutant alleles (p < 0.05) except for ash2 in the ash122 allele. Error bars represent standard error in microarray or semiquantitative RT-PCR analyses, respectively. (d) Comparison of genes that were upregulated or downregulated in ash2 and ash1 mutants with the genes preferentially expressed in the wing disc (WD) according to Klebes et al.  and in the body wall (BW) and wing hinge (W-H) according to Butler et al. . The full circle encompasses the total number of genes with valid log2 ratios in one or both of the ash2 alleles or in the ash1 allele for each comparison. The number of genes that were downregulated (green), upregulated (red) or showed no change in expression (yellow) over 1.5 times in each comparison is shown in brackets.