Figure 6
From: Deciphering the molecular machinery of stem cells: a look at the neoblast gene expression profile
Expression of selected genes differentially regulated in 30 Gy irradiated planarians and controls (whole mount in situ hybridization). Expression of the expressed sequence tag (EST) clone 32900731 (mRbAp48 [retinoblastoma-binding protein]) in (a) an untreated planarian and (b) a planarian 6 days after 30 Gy X-ray treatment. Expression of the EST clone 32901296 (H2A [histone family, member Z]) in (c) an untreated planarian and (d) a planarian 6 days after 30 Gy X-ray treatment. Expression of the EST clone 32903884 (Sam68-like mammalian protein 1) in (e) an untreated planarian and (f) a planarian 6 days after 30 Gy X-ray treatment. Expression of the EST clone 32902158 (TAF-Ibeta1) in (g) an untreated planarian and (h) a planarian 6 days after 30 Gy X-ray treatment. Expression of the EST clone 32903936 (HSP60 [heat shock protein 60]) in (i) an untreated planarian and (j) a planarian 7 days after 30 Gy X-ray treatment. Expression of the EST clone 32900868 (CIP-29) in (k) an untreated planarian and (l) a planarian 6 days after 30 Gy X-ray treatment. Expression of the EST clone 32899321 (AHNAK-related protein) in (m) an untreated planarian ((n) magnified section showing labeled epidermal cells) and (o) an intact planarian 6 days after 30 Gy X-ray treatment ((p) magnified section showing labeled epidermal cells). Scale bars: 500 μm in panels a-m and o; 30 μm in panel n; and 25 μm in panel p.