Figure 9

Identification of structural parameters that predict mRNA polysome loading in wild type and eif3h mutants. The average translation state of mRNAs was plotted separately for wild-type and eif3h mutant plants after subdividing the transcriptome (5,101 genes with polysome loading data and full-length cDNA support) according to the following parameters: (a) number of uAUGs in the 5' leader; (b) the eif3h mutation did not cause a global reduction in mRNA transcript levels for uORF-containing mRNAs; (c) length of the 5' leader in nucleotides (nt); (d) similar to (c), but leaders were classified into subgroups according to the number of uORFs. For clarity, we plotted only the difference in translation state between eif3h and wild type (colored lines). The stippled line denotes the percentage of leaders in each length class (cumulative). (e) Length of the 3' untranslated region. (f) Length of the protein-coding region of the main ORF. Standard errors are shown. The asterisk indicates a significant difference between the translation state in wild type and eif3h mutant (p < 0.05) according to a t-test (unpaired, two-tail).