Figure 8

uORF content and eIF3h-dependent polysome loading are correlated across functional categories of genes. Each datapoint represents a functional class of genes. The percentages of translationally up- or downregulated genes were plotted against the percentages of uAUG-containing genes in a given functional class. The four classes with the highest occurrence of uORFs and the two classes with the lowest occurrence are highlighted with pink and green circles, respectively. The left panels (a, c) focus on genes whose polysome loading is reduced in the eif3h mutant; whereas the right panels (b, d) focus on genes with stimulation of polysome loading. (a, b) Stringent cutoff; only genes with a two-fold or higher difference in polysome loading were considered. (c, d) Relaxed cutoff; genes with a 1.3-fold or higher difference in polysome loading were considered. Note that the correlation is apparent in each case.