Figure 3
Microarray analysis of polysome loading in the eif3h mutant. (a) Experimental design for the isolation of polysomal (PL) and non-polysomal (NP) RNAs. After sucrose density gradient centrifugation, samples were collected into 12 fractions. The integrity of the density gradient was confirmed by agarose gel electrophoresis and visualization of ribosomal RNAs with ethidium bromide. Microarray probes were generated from pooled samples as indicated. (b) Log2 transformed average translation states (TL = PL/NP) of the eif3h mutant were plotted against the data from wild-type plants. (c) Effects on polysome loading by the eif3h mutation (Log2 [TL]3h/[TL]WT) were not generally correlated with effects on transcript levels (Log2 [TC]3h/[TC]WT). An arbitrary two-fold cut-off was applied to highlight responsive genes (dotted lines). The number of genes affected both transcriptionally and translationally is very small (25 out of 6,238 genes for which reproducible data were available). Among them, the eIF3h mRNA is indicated by an arrow head.