Figure 3

Bulked segregant analysis of the trilobite locus employing anchored SNPs. Scatter plots provide SNP genotype data for a linked SNP (upper panel) and an unlinked SNP (lower panel). Data of individual embryo DNA is presented to the left and data of DNA pools is presented to the right. Under each plot is a further schematic representation of DNA sample genotype: 10 wild-type embryos, 50 mutant embryos, 1 wild-type embryo DNA pool, or 5 mutant embryo DNA pools. Homozygotes are shown in yellow or blue, heterozygotes are in red, and negative controls are in black. The data point of the wild-type pool at the linked SNP (top right) is intermediate between the position of a G/T heterozygote and G/G homozygote. Plot axes are in milli-polarization units, indicating relative dye terminator incorporation with single nucleotide primer extension detected by fluorescence polarization.