Analysis of the TLO gene family. (a) PCR validation of the telomeric TLO genes using a universal TLO primer coupled with a second primer that is specific for each chromosome arm. A putative TLO14 gene was identified on the 6R arm (see arrowhead). (b) Identification, by rtPCR, of two putative splicing variants using a 5' oligo that will recognize all TLO genes and two 3' primers with partial specificity for the two possible versions of the TLO8 transcripts. (c) Alignment of the amino acid sequences of the 14 TLO genes that are present in the latest genome assembly.