Table 1 Sequence of nucleotides and primers.

Splinkerette nucleotides

   HMSpAa

5'-CGA AGA GTA ACC GTT GCT AGG AGA GAC CGT GGC TGA ATG AGA CTG GTG TCG ACA CTA GTG G-3'

   HMSpBb-Sau3AI

5'-gat cCC ACT AGT GTC GAC ACC AGT CTC TAA (T)10C(A)7-3'

   HMSpBb-FatI

5'-cat gCC ACT AGT GTC GAC ACC AGT CTC TAA (T)10C(A)7-3'

Splinkerette PCR primers

   AB949new

5'-GCT AGC TTG CCA AAC CTA CAG GTG G-3'

   HM001

5'-GCC AAA CCT ACA GGT GGG GTC TTT-3'

   HMSp1

5'-CGA AGA GTA ACC GTT GCT AGG AGA GAC C-3'

   HMSp2

5'-GTG GCT GAA TGA GAC TGG TGT CGA C-3'

Primers for sequencing

   M13 forward primer

5'-CTG GCC GTC GTT TTA C-3'

   M13 reverse primer

5'-CAG GAA ACA GCT ATG AC-3'

RT-PCR primers

   Oligo-dT primer

5'-GGC CAC GCG TCG ACT AGT AC (T)₁₇-3'

   mCAT1-exon1-F

5'-GCG TGC GCC ATC CCC TCA GCT AGC A-3'

   mCAT1-exon3-R

5'-CGA TGA TGT AGG AGA GAA TCA GGT-3'

   mCAT1-exon4-F

5'-GTA CTT CAA GCG TGG CAA GAG-3'

   mCAT1-exon7-R

5'-CTG GTG GAA AGT GCG CAG AGA-3'

   mCAT1-exon8-F

5'-TCT CCT AGG CTC CAT GTT TCC C-3'

   mCAT1-exon12-R

5'-CTA TCA GCA TCC ACA CTG CAA A-3'

   LacZ-Gsp2-R

5'-ATG TGC TGC AAG GCG ATT AAG-3'

Genomic PCR primers

   Actb-exon4-up

5'-GTT TGA GAC CTT CAA CAC CCC-3'

   Actb-exon4-down

5'-GTG GCC ATC TCC TGC TCG AAG TC-3'

   zk274_in b-geo

5'-CGCCCCTGCGCTGACAGCCGGAACACGGCG-3'

   zk276RC_in b-geo

5'-AACTGCCAGCTGGCGCAGGTAGCAGAGCGG-3'