Repetitive DNA is associated with centromeric domains in Trypanosoma brucei but not Trypanosoma cruzi

Table 1 Summary of information available (GeneDB) on the AT-rich repeat domains

Chromosome number	Total size of repeat domain (kb)	Unit repeat size (bp)	AT content (%)	AT-rich array position orientated by flanking ORFs
1	5.5	~30	66	Tb927.1.3670 - Tb927.1.3750
2	8.0	29	66	Tb927.2.1470 - Tb927.2.1560
3	Unknown*	120	49	Tb927.3.3410 - Tb927.3.3430
4	3.5	147	61	Tb927.4.3740 - Tb927.4.3750
5	2.3	148	62	Tb927.5.600 - Tb927.5.610
6	Unknown^†	~30	71	Tb927.6.180 - Tb927.6.190
7	3.0	29	75	Tb927.7.6860 - Tb927.7.6870
8	Unknown^‡	147	59	Tb927.8.7740 - Tb927.8.7750
9	Unknown^§	147	60	Incomplete assembly
10	Unknown^¶	147	61	Incomplete assembly
11	Unknown^¥	147	61	Incomplete assembly

The AT-rich repeat domains are associated with the sites of etoposide-mediated Topo-II cleavage on T. brucei chromosomes 1-8 (Additional data file 4 and summarized in Figure 6). The flanking ORFs are those immediately adjacent to the repeat array. *BAC clone RPCI93-3K10 contains a large number of 120 bp repeats that span up to 30 kb (GeneDB). This region has not yet been closed. ^†The repeat array contains a gap of unknown size. ^‡There are 1.5 copies of this repeat next to a gap in the chromosome 8 sequence at position 2,233,104 bp. The unresolved array is estimated to be 5-9 kb (GeneDB). ^§Several chromosome 9 reads (approximately 700 bp) contain these repeats (for example, tryp_IXb-344h12.p1c, GeneDB). ^¶A 10 kb contig tryp_X-275g09.p1c (Tb10.v4.0130) and several chromosome 10 reads (approximately 700 bp) contain these repeats (for example, tryp_X-429d08.q1c, GeneDB). ^¥A 14 kb contig tryp_XI-974h12.q1k (Tb11.1750) and several chromosome 11 reads (approximately 900 bp) contain these repeats (for example, tryp_XI-991b09.q1k, GeneDB).

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