Figure 1

Representative mTLE-associated alternative splicing event identified using splice array technology. (a) Schematic of CLCN7 alternative splicing event associated with mTLE. Exons are shown in orange and intronic regions are shown in gray. (b) Data collected for the CLCN7 alternative splice event in control and mTLE brain tissue samples using the splice array technology (left) and quantitative rtPCR (qrtPCR, right). Data are presented as mean ± standard error of the mean; *p < 0.05 when compared to control, Student's t-test. (c) rtPCR confirmation of the pattern of transcript expression in brain tissue collected from ten subjects from each group. Both reference and variant transcript forms were amplified using the following primer sequences (indicated in the figure by arrows above mRNA transcripts): CLCN7F-GGCAAATACGCCCTGATG, CLCN7R-CTCAGCACGTCCACAATGAC.