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Figure 2 | Genome Biology

Figure 2

From: Analysis of proteomic profiles and functional properties of human peripheral blood myeloid dendritic cells, monocyte-derived dendritic cells and the dendritic cell-like KG-1 cells reveals distinct characteristics

Figure 2

Two-dimensional electrophoresis gels. Three representative two-dimensional gel images of (a) mDCs, (b) moDCs and (c) KG1 cells. Whole cell lysate protein (30 μg) was applied to immobilized pH gradient strips (pH 5-8), subjected to isoelectric focusing and separated on 10% to 20% polyacrylamide gel before silver staining. Images were analyzed using PDQuest and normalized by total quantity in valid spots. Highlighted spots were excised and protein identifications attempted using MALDI-TOF mass spectrometry. Boxed areas are shown in detail in Figure 3. Further gel information and protein identifications are shown in Table 2. The experiment was repeated three times (two times in the case of mDCs) with similar results.

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Genome Biology

ISSN: 1474-760X

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