Scheme for ICDS detection and resolution strategy. (a) ICDSs are detected within the genome by in silico analysis. The double daggers (‡) indicate the regions containing the identified frameshift. Upon resolution by sequencing and mass spectrometry analysis, the ICDSs can be classified as (b) true frameshifts or (c) sequencing errors. The hash symbol (#) indicates the region of the ORF containing the frameshift. The asterisks (*) indicate sites of corrected sequencing errors resulting in the reconstitution of a full-length ORF. The ORFs are depicted with arrows. The ORF may or may not be in the same frame. Proteins are represented by ellipses.