Figure 2

Post-translational modifications of histones H3 and H4 associated with the chromatin-binding proteins Suv39h1 and G9a. (a) Schematic representation of the purification protocols used to purify the HMT-histone complexes and crude histones. (b) Doubly immunopurified Suv39h1 complexes from chromatin extracts of 20 g of HeLa-Suv39h1 cells were resolved on a 4-12% gradient NuPAGE gel, run in MES buffer (Invitrogen), fixed, and stained with Colloidal blue. Lane 1, SeeBlue pre-stained molecular weight markers (Invitrogen); lane 2, Suv39h1 complex from chromatin fractions. (c) Amino-terminal lysine methylation of histones H3 and H4 associated with Suv39h1 or G9a proteins. (d) Post-translational modifications of histone H4 associated with Suv39h1 or G9a. Shown are the means of three independent experiments (± standard deviation).