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Figure 3 | Genome Biology

Figure 3

From: Application of the comprehensive set of heterozygous yeast deletion mutants to elucidate the molecular basis of cellular chromium toxicity

Figure 3

Chromium causes aggregation predominantly of proteins synthesized during chromium exposure. Cells were exposed to 0.1 mM CrO3 for 60 minutes, either (a) at the same time as or (b) after labeling with [35S]methionine for 60 minutes. The data show the relative enrichment of isotope in the aggregate fraction [cpm per μg aggregated protein, corrected for labeling efficiency (cpm per μg total protein)]. (b) Due to the natural turnover of labeled proteins during the post-labeling 60 minute incubation ± Cr, the data from this experiment were normalized with respect to the minus-Cr control from (a). (c) Aggregated protein as a proportion of total protein was determined after incubation of cells for 1 h in the absence or presence of 0.4 mM CrO3 and 10 μg ml-1 cycloheximide (CHX). All values are means ± standard error of the mean from three independent determinations.

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