Direct selection and phage display of a Gram-positive secretome

Table 1 Types of L. rhamnosus HN001 membrane-targeting sequences and distribution

Membrane-targeting signal in the insert	Bitopic or extracellular proteins	Polytopic integral membrane proteins	Total
Type I signal sequence	46*		46
Lipoprotein signal sequence	13		13
Amino-terminal transmembrane helix	12	6	18
Internal transmembrane helix	2	1	3
Multiple transmembrane helices		7	7
No recognizable membrane-targeting signals	2		2
Total	75	14	89

*Numbers refer to the number of secretome ORFs predicted to contain a particular type of membrane-targeting signal.

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