Figure 2

Conserved features of the CNB domain. A contrast hierarchical alignment showing conserved residues/motifs shared by the entire superfamily. The histograms above the alignments plot the strength of the selective constraints imposed at each position. Secondary structure is indicated directly above the aligned sequences with β-strands indicated by their number designations (that is, 1-7 correspond to the β1-β7 strands, respectively) and helices by their letter designations. The leftmost column of each alignment shows the sequences used in the display alignment. See Materials and methods for sequence identifiers. The background alignment of all CNB domain containing sequences are shown indirectly via the consensus patterns and corresponding weighted residue frequencies ('wt_res_freqs') below the display alignment. (Such sequence weighting adjusts for overrepresented families in the alignment.) The residue frequencies are indicated in integer tenths where, for example, a '5' indicates that the corresponding residue directly above it occurs in 50-60% of the weighted sequences. Biochemically similar residues are colored similarly with the intensity of the highlighting proportional to how strikingly foreground residues contrast with background residues.