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Figure 7 | Genome Biology

Figure 7

From: Broad network-based predictability of Saccharomyces cerevisiaegene loss-of-function phenotypes

Figure 7

Network-guided extension of a genetic screen. Guilt-by-association (GBA) was applied to predict essential yeast genes whose disruption resulted in elongated yeast cells, based on the genes' network connectivity to a seed set of 77 nonessential genes already known to cause cell elongation when deleted [4]. (a) Five examples of successful predictions, observed in yeast strains carrying tetracycline downregulatable conditional alleles [47] of the essential genes TAF9, MED6, MED7, SWI1, and RPO21. In contrast, conditional downregulation of an unrelated essential gene, SCM3, caused no such cell elongation. (b) Sixteen out of 33 tested essential genes (yellow circles) showed elongated cell phenotypes on the basis of their connections to the seed set genes (green circles), with particular enrichment for genes associated with RNA polymerase II transcriptional initiation and the mediator complex. The color of the edge between two genes indicates the source of evidence supporting the functional link: thick black, multiple types of evidence; blue, affinity purification/mass spectrometry; green, literature mining by co-citation; cyan, gene neighbors or tertiary structure; pink, literature curated physical interaction; and red, genetic interaction.

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