Cdc10-GFP protein dynamics throughout spore germination. Purified spores containing GFP-tagged Cdc10 (prepared from strain DS38) were plated and synthetic minimal medium was added to allow spore germination. Time lapse microscopy was carried out using a Deltavision RT microscope system with OAI Scan command and the results deconvolved. (a-c) Spore germination before the appearance of the first bud. Images were monitored at 60× magnification using (a) FITC (excitation 490 and emission 526) filter, (b) RD-TR-Cy3 (excitation 555 and emission 617) filter and (c) merged pictures of (a) and (b). (d) The first mitotic cell cycle was monitored at 100× magnification using a FITC filter. In yellow is the time following the addition of synthetic minimal medium in hours and minutes.