Table 7 Primer sequences
Gene | Primer | Sequence | Annealing Tm (C) | MgCl2 conc. (μM) | Product size (bp) |
|---|---|---|---|---|---|
HPRT | Sense Antisense | 5'-CTTGCGACCTTGACCATCTTTGGA-3' 5'-GGCGTCGTGATTAGTGATGATGAACC-3' | 58 | 1.5 | 467 |
GATA-2 | Sense Antisense | 5'-TATGTGCCGGCGGCTGCCCACGACTACA-3' 5'-GGCTCTTCTGGCGGCCGACAGTCTT-3' | 60 | 1.5 | 280 |
SCL | Sense Antisense | 5'-GAAGTGCTCCCCTCTGAAAGTT-3' 5'-GGCTATCTCTCCTCTGACCTCG-3' | 58 | 1.5 | 319 |
β-Cluster globin genes | Sense Antisense | 5'-GTYTACCCHTGGACCCAGA-3' 5'-GCAGCTTGTCACAGTGCAG-3' | 56 | 2 | 190 |
OCT4 | Sense Antisense | 5'-GAAGGTATTCAGCCAAACGAC-3' 5'-GTTACAGAACCACACTCGGA-3' | 55 | 2 | 315 |
Nanog | Sense Antisense | 5'-TGCAAATGTCTTCTGCTGAGAT-3' 5'-GTTCAGGATGTTGGAGAGTTC-3' | 55 | 2 | 285 |
Rex-1 | Sense Antisense | 5'-TGACAGGCAAGAAGCTTCCG-3' 5'-GCGTACGCAAATTAAAGTCCAGA-3' | 55 | 2 | 350 |