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Table 1 Characterization of hESCs and BCs by immunocytochemistry and Affymetrix arrays

From: GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silicodissection of mixed phenotypes

  

Level I

Level II

Level III

 

IC

BC direct

Epithelium

Leukocytes

Endothelium

Stromal

GATA1

+

+

+

+

+

+

GATA2

+

+

+

--

--

--

CD71

+

+

+

+

--

+

EPO-R

+

--

+

+

+

+

TPO-R

+

--

--

--

--

+

LMO2

+

+

+

+

--

+

β-Catenin

+

--

--

--

--

--

EGR1

+

--

--

--

--

--

Integrin α4

+/-

--

--

--

--

--

Integrin β1

+/-

--

--

--

--

--

VE-cadherin

--

--

--

+

--

--

E-cadherin

--

--

--

--

--

--

KDR

--

--

--

--

--

--

PECAM (CD31)

--

--

--

+

--

--

CD34

--

--

--

+

--

--

CD41

--

--

CD41b

--

CD41b

CD41b

CD43

--

--

--

+

+

+

  1. The reliability of the microarray data generated from our multi-comparison analysis is demonstrated by the consistent identification of a set of genes among multiple comparisons, of which a subset of genes were confirmed by immunocytochemistry. For immunochemistry (IC): +, moderate to strong staining; -, negative staining; +/-, very weak staining. For Affymetrix arrays:+, detected as up-regulated in BCs; --, not detected as up-regulated in BCs.