Figure 4

Ingenuity pathway analysis shows a network of genes expressed in EB and BC data sets from the level II analysis. This network contains nodes (genes/gene products) and edges (relationships between the nodes). The shaded genes, known as focus genes, were identified by microarrays and are the starting point to generate the network. The asterisks indicate that duplicates were identified in each dataset. The EB data set contains a network of genes (VEGF, GATA4, BMP4) that are interconnected and involved in blood vessel development (VEGF), heart development (GATA4), and cellular development (BMP4). The BC data set identified a network of genes involved in cardiovascular development (SHH, RARB, TBX5, WNT11) acting through GATA4. The intensity of the node color indicates the degree of expression. Nodes are displayed using various shapes that represent the functional class of the gene product (diamond-enzymes, ovals-transcription factors, triangles-kinase, circles-others). A solid line indicates a direct interaction while a dashed line indicates an indirect interaction. A line without an arrowhead indicates binding and a plus sign indicates that othernetworks contain this gene product.