Determinants of close non-adjacently conserved pairs versus distant adjacently conserved pairs. The difference between the ratio of determinant values of non-adjacently conserved genes in a close species to S. cerevisiae (C. glabrata) and those adjacently conserved in a distant species (A. gossypii) is plotted in red for each predictor (line between points to help visualization). This ratio is defined as the quotient between the corresponding values of the close (distant) pairs and those of the adjacently conserved pairs in the close species, that is, C. glabrata. We also plotted the null behavior obtained by random sampling of the combined group, close and distant, preserving group size, 10,000 times (mean, continuous blue line, ±2 standard deviations, dashed blue lines). Behavior was qualitatively robust for the cex, igd, and let predictors when using S. castelli and K. lactis as close/distant comparator (Additional data file 1).