Figure 8

Expected fraction of breakpoints, given five-fold redundant clone coverage, captured by ≥N clones with the distance between breakpoint and clone terminus larger than detection cutoff. The plot shows detection profiles for 150 kb and 220 kb clones. The plot illustrates the benefit of redundant coverage and of using clones with larger inserts - for a given detection cutoff, a breakpoint is captured by significantly more clones on average. The detection sensitivity (Figure 4) needs to be applied to the fraction of breakpoints on this plot (for example, 80% of breakpoints found in ≥2 clones within 50 kb of the ends of the clone; assuming 2 digests, 95% of 50 kb regions can be aligned (Figure 4); therefore, 80% × 0.95 = 76% of breakpoints are expected to be detected in these conditions).