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Table 8 Sequences of the primers used for validation of microarray results by real-time PCR

From: Gene expression profiling of Hfe-/-liver and duodenum in mouse strains with differing susceptibilities to iron loading: identification of transcriptional regulatory targets of Hfe and potential hemochromatosis modifiers

Gene GeneBank accession Forward primer Reverse primer Amplification efficiency
Hprt NM_013556 5'-CTG GTT AAG CAG TAC AGC CCC AA-3' 5'-CAG GAG GTC CTT TTC ACC AGC-3' 1.99
Aox1 NM_009676 5'-CAC CCT GTA TTC ATC TAA GGG CCT-3' 5'-CAC TGC ATC ATG GAT GGC AA-3' 1.92
Ftl1 NM_01024 5'-GGA GAA GAA CCT GAA TCA GGC C-3' 5'-GGT TGC CCA TCT TCT TGA TGA G-3' 2.00
Fpn1 NM_016917 5'-CAT TGC TGC TAG AAT CGG TCT T-3' 5'-GCA ACT GTG TCA CCG TCA AAT-3' 1.97
Hmox1 NM_010442 5'-CAG ATG GCG TCA CTT CGT CA-3' 5'-CTC TGC AGG GGC AGT ATC TTG-3' 2.00
Vanin1 NM_011704 5'-GGC TGC ACA CCG TGG AAG-3' 5'-GGT AAA AGC CGT GTC CAC TGA A-3' 1.90
Por NM_008898 5'-GCC TCG TCG TCT AAG GTC CA-3' 5'-GAC TTC GCT TCA TAC TCC ACA GC-3' 1.99
Cpt1a NM_013495 5'-GAC CCC ACA ACA ACG GCA G-3' 5'-ATG GCG AGG CGG TAC AGG T-3' 2.00
Aco2 NM_080633 5'-GAC CAT TCA AGG CCT GAA GG-3' 5'-ACG CAC TTC AGA GGC TTT CC-3' 2.00
Cyp7a1 NM_007824 5'-GCT CTG GAG GGA ATG CCA T-3' 5'-CCG CAG AGC CTC CTT GAT G-3' 2.00
Hsd3b5 NM_008295 5'-AGA GGA ATT GTC CAA GCT GCA-3' 5'-TGT GGA TGA CAG CAG ACA TGC-3' 1.99
Hfe2 NM_027126 5'-ACC ACC ATC CGG AAG ATC ACT-3' 5'-AAG GCT GCA GGA AGA TTG TCC-3' 2.00
Hamp1 AF_503444 5'-AAG CAG GGC AGA CAT TGC GAT-3' 5'-CAG GAT GTG GCT CTA GGC TAT GT-3' 1.98
Hamp2 AY_232841 5'-AAG CAG GGC AGA CAT TGC GAT-3' 5'-GGA TGT GGC TCT AGG CTC TCT ATT-3' 2.00
Usf2 NM_011680 5'-ATG GAA CCA GAA CTC CTC GAG A-3' 5'-CCG TTC CAC TTC ATT GTG CTG-3' 1.93
Lcn2 NM_008491 5'-TCT GTC CCC ACC GAC CAA T-3' 5'-CCA GTC AGC CAC ACT CAC CAC-3' 1.99
Sfxn2 NM_053196 5'-CGC ACA AGT GGT TAT CTC TCG G-3' 5'-CCA TGA TGA CAG GCA ACA GGA-3' 1.99
Alas2 NM_009653 5'-TGG AAC TCT TGG CAA GGC C-3' 5'-CAA GTC CCG AGT GCT GGC T-3' 1.99
Slc25a37 NM_026331 5'-GAG CAC TCC ATC ATG TAC CCG-3' 5'-TGG ATT CAA ACT CTG CAT CCG-3' 2.00
Abcg2 NM_011920 5'-TTG GCT GTC CTG GCT TCA GTA C-3' 5'-CAA AGC TGT GAA GCC ATA TCG A-3' 1.99
Cybrd1 AF_354666 5'-GCA GCG GGC TCG AGT TTA-3' 5'-TTC CAG GTC CAT GGC AGT CT-3' 1.98
Slc39a4 NM_028064 5'-GCG ACT GAG AGC AGA GCT GA-3' 5'-GTT GTG TAC CGC GTC GCC-3' 2.00
Mucin3 NM_355711 5'-TCG TGT TCT CCA TCC GCT TC-3' 5'-GAC ACT CTG GAC CGT TGC CT-3' 1.99
Lcn13 NM_153558 5'-TGT TTG TGC CAG AGA TCG GAG-3' 5'-GCT GGC TCA GCT GTT GCA G-3' 1.95
Fmo3 NM_008030 5'-GGA ACT TGC ACT TTG CCT TCT G-3' 5'-GGT GGT GCT ATT GCC ATA CCA-3' 1.96
Clca4 NM_139148 5'-GCC GTC ATA GAA GCT GAG AGT GG-3' 5'-AGC ACC TGC CCC GTT GTC-3' 2.00
  1. Hfe-/- and Hfe+/+ mice (five males per group) were killed at age 7 weeks. Blood was removed and plasma lipid levels were determined by chromatography. Results are expressed as mean ± standard deviation in each group. P values for comparisons of plasma lipid levels between Hfe-/- and Hfe+/+ mice of each strain were obtained by Student's t-test. HDL, high-density lipoprotein.