Skip to main content

Table 8 Sequences of the primers used for validation of microarray results by real-time PCR

From: Gene expression profiling of Hfe-/-liver and duodenum in mouse strains with differing susceptibilities to iron loading: identification of transcriptional regulatory targets of Hfe and potential hemochromatosis modifiers

Gene

GeneBank accession

Forward primer

Reverse primer

Amplification efficiency

Hprt

NM_013556

5'-CTG GTT AAG CAG TAC AGC CCC AA-3'

5'-CAG GAG GTC CTT TTC ACC AGC-3'

1.99

Aox1

NM_009676

5'-CAC CCT GTA TTC ATC TAA GGG CCT-3'

5'-CAC TGC ATC ATG GAT GGC AA-3'

1.92

Ftl1

NM_01024

5'-GGA GAA GAA CCT GAA TCA GGC C-3'

5'-GGT TGC CCA TCT TCT TGA TGA G-3'

2.00

Fpn1

NM_016917

5'-CAT TGC TGC TAG AAT CGG TCT T-3'

5'-GCA ACT GTG TCA CCG TCA AAT-3'

1.97

Hmox1

NM_010442

5'-CAG ATG GCG TCA CTT CGT CA-3'

5'-CTC TGC AGG GGC AGT ATC TTG-3'

2.00

Vanin1

NM_011704

5'-GGC TGC ACA CCG TGG AAG-3'

5'-GGT AAA AGC CGT GTC CAC TGA A-3'

1.90

Por

NM_008898

5'-GCC TCG TCG TCT AAG GTC CA-3'

5'-GAC TTC GCT TCA TAC TCC ACA GC-3'

1.99

Cpt1a

NM_013495

5'-GAC CCC ACA ACA ACG GCA G-3'

5'-ATG GCG AGG CGG TAC AGG T-3'

2.00

Aco2

NM_080633

5'-GAC CAT TCA AGG CCT GAA GG-3'

5'-ACG CAC TTC AGA GGC TTT CC-3'

2.00

Cyp7a1

NM_007824

5'-GCT CTG GAG GGA ATG CCA T-3'

5'-CCG CAG AGC CTC CTT GAT G-3'

2.00

Hsd3b5

NM_008295

5'-AGA GGA ATT GTC CAA GCT GCA-3'

5'-TGT GGA TGA CAG CAG ACA TGC-3'

1.99

Hfe2

NM_027126

5'-ACC ACC ATC CGG AAG ATC ACT-3'

5'-AAG GCT GCA GGA AGA TTG TCC-3'

2.00

Hamp1

AF_503444

5'-AAG CAG GGC AGA CAT TGC GAT-3'

5'-CAG GAT GTG GCT CTA GGC TAT GT-3'

1.98

Hamp2

AY_232841

5'-AAG CAG GGC AGA CAT TGC GAT-3'

5'-GGA TGT GGC TCT AGG CTC TCT ATT-3'

2.00

Usf2

NM_011680

5'-ATG GAA CCA GAA CTC CTC GAG A-3'

5'-CCG TTC CAC TTC ATT GTG CTG-3'

1.93

Lcn2

NM_008491

5'-TCT GTC CCC ACC GAC CAA T-3'

5'-CCA GTC AGC CAC ACT CAC CAC-3'

1.99

Sfxn2

NM_053196

5'-CGC ACA AGT GGT TAT CTC TCG G-3'

5'-CCA TGA TGA CAG GCA ACA GGA-3'

1.99

Alas2

NM_009653

5'-TGG AAC TCT TGG CAA GGC C-3'

5'-CAA GTC CCG AGT GCT GGC T-3'

1.99

Slc25a37

NM_026331

5'-GAG CAC TCC ATC ATG TAC CCG-3'

5'-TGG ATT CAA ACT CTG CAT CCG-3'

2.00

Abcg2

NM_011920

5'-TTG GCT GTC CTG GCT TCA GTA C-3'

5'-CAA AGC TGT GAA GCC ATA TCG A-3'

1.99

Cybrd1

AF_354666

5'-GCA GCG GGC TCG AGT TTA-3'

5'-TTC CAG GTC CAT GGC AGT CT-3'

1.98

Slc39a4

NM_028064

5'-GCG ACT GAG AGC AGA GCT GA-3'

5'-GTT GTG TAC CGC GTC GCC-3'

2.00

Mucin3

NM_355711

5'-TCG TGT TCT CCA TCC GCT TC-3'

5'-GAC ACT CTG GAC CGT TGC CT-3'

1.99

Lcn13

NM_153558

5'-TGT TTG TGC CAG AGA TCG GAG-3'

5'-GCT GGC TCA GCT GTT GCA G-3'

1.95

Fmo3

NM_008030

5'-GGA ACT TGC ACT TTG CCT TCT G-3'

5'-GGT GGT GCT ATT GCC ATA CCA-3'

1.96

Clca4

NM_139148

5'-GCC GTC ATA GAA GCT GAG AGT GG-3'

5'-AGC ACC TGC CCC GTT GTC-3'

2.00

  1. Hfe-/- and Hfe+/+ mice (five males per group) were killed at age 7 weeks. Blood was removed and plasma lipid levels were determined by chromatography. Results are expressed as mean ± standard deviation in each group. P values for comparisons of plasma lipid levels between Hfe-/- and Hfe+/+ mice of each strain were obtained by Student's t-test. HDL, high-density lipoprotein.