Skip to main content

Table 5 Changes in expression of genes involved in iron metabolism

From: Gene expression profiling of Hfe-/-liver and duodenum in mouse strains with differing susceptibilities to iron loading: identification of transcriptional regulatory targets of Hfe and potential hemochromatosis modifiers

Gene Protein Major biochemical activity Role Organ S-score
      D2 KO versus D2 WT B6 KO versus B6 WT D2 WT versus B6 WT
Iron storage
   Ftl1 Ferritin L chain Fe mineralization Cytosolic storage Liver +1.70 +2.24 NS
Iron transport
   Slc40a1 Ferroportin Membrane transporter Cellular export Liver +3.18 +4.89 +3.94
   Abcg2 Bcrp Membrane transporter Possible mitochondrial heme export Liver +2.97 NS NS
   Sfxn2 Sideroflexin2 Membrane transporter Mitochondrial traffic Liver -2.38 -2.16 NS
   Slc25a37 Mitoferrin Membrane transporter Mitochondrial traffic Liver NS +2.28 NS
   Lcn2 Lipocalin2 Siderophore iron binding Traffic of siderophore-bound iron Liver -2.91 NS NS
Receptors
   Tfrc Transferrin receptor1 Transferrin binding Transferrin iron uptake Duodenum -2.07 NS NS
   Lrp1 LRP/CD91 Hemoplexin receptor Hemoplexin uptake Liver NS -2.03 NS
Regulators
   Ireb2 IRP2 RNA binding Control of cellular iron Duodenum +2.26 NS -2.64
   Hamp1 Hepcidin 1 Ferroportin binding Control of systemic iron Liver -6.27 NS -3.57
   Hamp2 Hepcidin 2 ? ? Liver -3.40 NS +3.36
   Hfe HFE TfR1 binding ? Liver -7.96 -8.96 -3.16
     Duodenum -5.70 -7.32 NS
   Hfe2 HJV Neogenin binding Control of hepcidin expression Liver NS -2.01 NS
   Fxn Frataxin Iron binding Chaperon for Fe-S synthesis Liver NS NS -3.09
   Smad4 Smad4 Transcription factor Control of hepcidin expression Liver NS NS -3.59
     Duodenum NS NS -5.05
   Usf2 Usf2 Transcription factor Control of hepcidin expression Liver NS +2.08 NS
Oxidoreductases
   Cybrd1 Dcytb Fe(III) reduction Facilitates duodenal transport by DMT1 Duodenum +2.97 NS NS
  1. Iron metabolism genes are cited in this table where significant expression variations in Hfe-/- mice (knockout [KO]) or expression differences between wild-type (WT) strains were detected. S-scores were obtained as described in Material and Methods and are proportional to fold changes. Positive S-scores indicate that the genes are more highly expressed in KO than in WT mice, or in WT D2 than in WT B6 mice. NS, not significant.