Gene expression profiling of Hfe-/-liver and duodenum in mouse strains with differing susceptibilities to iron loading: identification of transcriptional regulatory targets of Hfe and potential hemochromatosis modifiers

Table 2 Main genes regulated by Hfe deficiency in liver and pertaining to enriched functional categories related to response to oxidative stress

Gene	Protein	S-score
		D2 KO versus WT	B6 KO versus WT	D2 WT versus B6 WT
Glutathione metabolism pathway
Gclc	Glutamate-cysteine ligase, catalytic subunit	3.68	4.74	3.24
Gclm	Glutamate-cysteine ligase, modifier subunit	NS	4.11	NS
Gss	Glutathione synthetase	NS	2.15	NS
Gsta2	Glutathione S-transferase alpha2	8.83	9.10	NS
Gsta3	Glutathione S-transferase alpha3	2.44	2.24	NS
Gsta4	Glutathione S-transferase alpha4	4.64	5.99	4.35
Gstm1	Glutathione S-transferase mu1	NS	2.65	NS
Gstm3	Glutathione S-transferase mu3	3.04	4.04	2.56
Gstm6	Glutathione S-transferase mu6	3.36	2.26	NS
UDP glucuronosyltransferase activity
Ugt2b1	UDP glucuronosyltransferase 2B1	3.72	NS	NS
Ugt2b5	UDP glucuronosyltransferase 2B5	2.56	3.91	NS
Ugt2b34	UDP glucuronosyltransferase 2B34	NS	2.25	NS
Ugt2b35	UDP glucuronosyltransferase 2B35	2.56	3.91	NS
Ugt2b36	UDP glucuronosyltransferase 2B36	4.66	NS	-4.89
Mono-oxygenase activity
Cyp1a2	Cytochrome P450 1A2	3.14	2.60	3.49
Cyp2c29	Cytochrome P450 2C29	NS	2.79	4.84
Cup2c44	Cytochrome P450 2C44	3.07	NS	-5.89
Cyp2c55	Cytochrome P450 2C55	3.94	6.22	4.43
Cyp2c70	Cytochrome P450 2C70	5.55	4.70	4.17
Cyp2j6	Cytochrome P450 2J6	2.70	NS	NS
Cyp2j9	Cytochrome P450 2J9	NSD	2.91	2.14
Cyp2u1	Cytochrome P450 2U1	3.50	NS	NS
Fmo5	Flavin mono-oxygenase 5	2.12	NS	NS
Iron ion binding
Ftl1	Ferritin light chain 1	1.70	2.24	NS
Slc40a1	Ferroportin	3.18	4.89	3.94
Hmox1	Heme oxygenase 1	5.27	2.40	-3.66
Blvrb	Biliverdin reductase (for information)	2.50	3.06	NS
Vnn1	Vanin 1 (for information)	-4.27	-2.73	2.69

S-scores were obtained as described in Materials and methods and are proportional to fold changes. Positive S-scores indicate that the genes are more highly expressed in knockout (KO) than in wild-type (WT) mice, or in WT D2 than in WT B6 mice. NS, not significant; UDP, Uridine 5'-diphospho.

ISSN: 1474-760X