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Table 2 Summary of data from quantitative RT-PCR on the S. solfataricus genes tfb3, cdc6-2, xpb1, dpoII and ssb

From: Responses of hyperthermophilic crenarchaea to UV irradiation

  

CP*

UV/control expression ratio

Protein

Sso no.

UV (120 min)

Control (120 min)

30 min

60 min

90 min

120 min

TFB1

0446

14.7 ± 0.21

14.5 ± 0.06

0.76

1.02

1.37

0.92

TFB2

0946

21.4 ± 0.21

20.2 ± 0.15

0.71

0.54

0.83

0.49

TFB3

0280

12.5 ± 0.06

16.3 ± 0.06

2.2

14.4

24.2

12.6

Cdc6-2

0771

12.7 ± 0.06

15.8 ± 0

1.8

7.4

6.6

4.7

XPB1

0959

13.8 ± 0.1

13.6 ± 0.12

0.80

1.07

1.05

0.92

DpoII

1459

11.1 ± 0.12

13.7 ± 0.06

1.0

3.07

4.41

3.44

SSB

2364

9.9 ± 0.12

9.8 ± 0.23

0.98

0.88

0.92

0.90

  1. *CP: the cycle number at which the fluorescence crossed the threshold during PCR. The lower the CP, the more abundant the transcript. CP values are means of triplicate experiments with standard deviations shown. The expression ratio was calculated according to the method of [50].