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Table 2 Summary of data from quantitative RT-PCR on the S. solfataricus genes tfb3, cdc6-2, xpb1, dpoII and ssb

From: Responses of hyperthermophilic crenarchaea to UV irradiation

   CP* UV/control expression ratio
Protein Sso no. UV (120 min) Control (120 min) 30 min 60 min 90 min 120 min
TFB1 0446 14.7 ± 0.21 14.5 ± 0.06 0.76 1.02 1.37 0.92
TFB2 0946 21.4 ± 0.21 20.2 ± 0.15 0.71 0.54 0.83 0.49
TFB3 0280 12.5 ± 0.06 16.3 ± 0.06 2.2 14.4 24.2 12.6
Cdc6-2 0771 12.7 ± 0.06 15.8 ± 0 1.8 7.4 6.6 4.7
XPB1 0959 13.8 ± 0.1 13.6 ± 0.12 0.80 1.07 1.05 0.92
DpoII 1459 11.1 ± 0.12 13.7 ± 0.06 1.0 3.07 4.41 3.44
SSB 2364 9.9 ± 0.12 9.8 ± 0.23 0.98 0.88 0.92 0.90
  1. *CP: the cycle number at which the fluorescence crossed the threshold during PCR. The lower the CP, the more abundant the transcript. CP values are means of triplicate experiments with standard deviations shown. The expression ratio was calculated according to the method of [50].