Figure 1
From: Responses of hyperthermophilic crenarchaea to UV irradiation
Experimental design and DNA damage. (a) Illustration of the experiment design. After growth to early log phase, cells were placed in Petri dishes and exposed to 200 J/m2 UV irradiation, then allowed to recover in the dark at 80°C. Arrows indicate sampling time points. (b) Optical density measurement of S. solfataricus UV-treated (red) and control (blue) cultures. Cells were exposed to UV at time zero. (c) Generation and removal of CPDs in chromosomal DNA. DNA damage was measured by ELISA using a primary antibody specific for CPDs. Data were normalized so that the signal at time zero (immediately after UV irradiation) represented 100% unrepaired CPDs. Each data point represents the mean from six experimental replicates and error bars show the standard deviation of each data set.