Testosterone does not alter Scube1 mRNA levels or expression pattern. (a) Whole-mount RNA in situ hybridization of ventral prostates (VPs) grown in vitro for 6 days in the absence (-T) or presence (+T) of testosterone. (b) Quantitative RT-PCR for Scube1 and FGF10 mRNAs in VPs grown in vitro with/without testosterone. VPs were cultured in the absence of testosterone for 3 days followed by an incubation of 24 hours in the presence or absence of testosterone. (c) Northern analysis for Scube1 mRNA on P0 female urogenital tracts treated in vitro with testosterone for 6 hours and 24 hours. VSU, ventral mesenchymal pad, smooth muscle, urethra.