Expression of Scube1 mRNA in rat P0 tissues and during prostate development. (a) Northern blot analysis of Scube1 mRNA levels in P0 tissues; highest levels of Scube1 transcripts were observed in testis and ovary. Brain, ventral prostate (VP), bladder, and kidney showed moderate Scube1 expression. Kidney and lung showed very low expression, and liver was negative. (b) Expression of Scube1 in male and female urogenital tract (UGT) at E17.5, and subsequent expression the VP at P0, P4, P10, P28, and adult.