Localization of Scube1 to the inductive mesenchyme (VMP) of female UGT. (a) Comparison of Scube1 transcript levels in ventral mesenchymal pad (VMP) and VSU (VMP, smooth muscle and urethral epithelium) using serial analysis of gene expression (SAGE) and quantitative RT-PCR. Red bars represent the SAGE data, and yellow bars represent the quantitative RT-PCR data (normalized to TBP levels). Scube1 mRNA was found to be enriched in the VMP by both SAGE and quantitative RT-PCR analyses. (b) Northern analysis showing a twofold enrichment of Scube1 mRNA levels in the VMP compared with the VSU. (c) RNA whole-mount in situ hybridization of Scube1 in P0 female UGT; anti-sense probe is at top of panel and sense is at the bottom of the panel. Scube1 transcripts localized to the VMP, and were not observed in smooth muscle (SM) and urethral epithelium (URE).