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Figure 8 | Genome Biology

Figure 8

From: A genetic code alteration generates a proteome of high diversity in the human pathogen Candida albicans

Figure 8

Ambiguous CUG decoding generates phenotypic diversity. (a) Candida albicans control cells (pUA12) grew in agar plates as white, smooth, or slightly rough colonies. (b) Expression of the Saccharomyces cerevisiae tRNALeu (pUA15) in C. albicans resulted in 88.9 ± 4.3% morphogenesis (data not shown), with appearance of an array of morphologic phenotypes. Morphology variation was characterized by appearance of large sectors containing opaque cells and aerial hyphae and by formation of unusual morphologic structures in the colonies. (c) Colonies with homogeneous morphology isolated from sectors of colonies shown in panel b. In panels a and b, phenotypic variability was determined on agar plates after 7 days of growth, considering all morphologic changes that deviated from the white smooth phenotype, which is characteristic of C. albicans wild-type cells.

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