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Table 3 Putative Hymenoptera-specific genes

From: An annotated cDNA library and microarray for large-scale gene-expression studies in the ant Solenopsis invicta

  Solenopsis invicta assembled sequence1 Blast statistics Apis mellifera sequence Confidence7
Identifier (length) Span Frame ORF2 (bp) I3 Exp4 Bit-score E-value Linkage Group Span Strand ORF2 (bp) Est5 Annotated gene6  
SI.CL.8.cl.881.Contig1 (724 bp) 509-640 2 300   272 1.24E-18 6 2701427-2701558 + 429   Ab initio prediction ***
SI.CL.8.cl.843.SiJWH04BDO2.scf (730 bp) 582-761 3 147   210 1.99E-12 NW_001254419.8 44307-44486 - 147 Near NH homology. GB18184-PA on reverse strand **
SI.CL.19.cl.1938.Contig1 (835 bp) 21-323 3 372 T 212 1.43E-12 6 1145090-1145392 - 429   Ab initio prediction. Near GB12791-PA on reverse strand ***
SI.CL.19.cl.1953.SiJWC11BBX.scf (613 bp) 81-215 3 555   166 5.08E-08 8 5253595-5253729 - 372 GB14543-PA. Near NH homology on reverse strand *
  306-416      87 4.5E-15   5252894-5253094   306    
  435-635      200    5253189-5253299   318    
SI.CL.23.cl.2326.Contig1 (632 bp) 413-577 2 219   291 1.33E-20 11 8022183-8022347 + 480   Ab initio prediction ***
SI.CL.26.cl.2688.Contig1 (859 bp) 60-131 39 87   98 9.74E-15 9 10421877-10421948 - 549 Ab initio prediction. Near NH homology on reverse strand **
  119-256 29 558    186    10421751-10421888      
SI.CL.33.cl.3311.Contig1 (710 bp) 228-359 3 189   258 3.07E-17 14 8634060-8634191 - 132 Near ab initio prediction. Near NH homology on reverse strand *
SI.CL.33.cl.3384.Contig1 (469 bp) 229-327 19 264 T,S 160 3.11E-13 14 3770768-3770866 - 231   Ab initio prediction ***
  362-454 29 180 S   104    3770649-3770741   186    
SI.CL.35.cl.3595.Contig1 (415 bp) 123-398 3 342   301 5.97E-22 NW_001261806.8 12471-12746 + 327   Ab initio prediction ***
SiJWA02BAZ2.scf (600 bp) 374-469 2 261   193 2.13E-15 5 9909503-9909598 + 627 Near GB15931-PA and NH homology on reverse strand *
  533-604      98    9909356-9909427      
SiJWA03CAW.scf (666 bp) 49-144 1 96    120 2.1E-16 NW_001259848.8 47860-47955 + 99 GB10007-PA on reverse strand ***
  136-297   117    182    47704-47865   726    
SiJWA12ACK.scf (212 bp) 137-268 29 69   264 1.42E-19 3 5151467-5151598 + 162 Near ab initio prediction and NH homology on reverse strand **
  63-143 39 72    69    5151391-5151471   189    
SiJWB12BCQ.tag5_B12_04.scf (754 bp) 121-369 1 354   254 1.1E-16 7 5620128-5620376 + 336   Ab initio prediction on reverse strand ***
SiJWC11BAT.scf (342 bp) 189-278 3 228   160 3.98E-17 14 8645843-8645932 + 162 Near ab initio prediction and homology **
  282-368      123 6.41E-14   8645754-8645840   117    
SiJWE02BBO2.scf (865 bp) 714-863 3 129   243 1.26E-15 6 4850974-4851123 - 354   Near ab initio prediction on reverse strand **
SiJWF07BCC.tag5_F07_11.scf (799 bp) 329-529 2 96   196 6.59E-11 3 6205208-6205408 - 108   Near NH homology. Ab initio prediction on reverse strand **
SiJWG01BDU2.scf (759 bp) 21-227 3 102   354 1.23E-26 2 9618145-9618351 + 171 GB12576-PA and NH homology on reverse strand *
SiJWG03ACB.scf (623 bp) 172-609 1 471   558 4.63E-47 10 2344965-2345402 + 1440 GB19005-PA ***
SiJWH02AAN.scf (469 bp) 100-294 1 102   341 1.32E-30 12 281374-281568 - 294   - ***
  28-105   69    104    281564-281641   207    
SiJWH05BDPR5A08.scf (658 bp) 580-657 1 78   161 1.1E-15 10 2890267-2890344 + 159 Near ab initio prediction **
SiJWH05BDV2.scf (517 bp) 204-353 3 198   237 4.87E-15 5 6704423-6704572 + 174   Ab initio prediction **
SiJWH08AAT.scf (653 bp) 76-162 1 60   141 4.53E-20 5 1169177-1169263 + 84 Near ab initio prediction and NH homology *
  151-195   102    75 4.52E-13   1169261-1169305   69    
SiJWH08ADY.scf (563 bp) 236-496 2 327   312 1.32E-22 12 4477772-4478032 - 432   GB16574-PA ***
  1. 1Solenopsis invicta assembled sequences that show no significant similarity to any known non-hymenopteran sequence (E > 1), but high similarity to a region of the honey bee genome (E < e-10). 2Length in base-pairs of the largest overlapping in-frame open reading frame. 3In-frame Interproscan annotation of fire ant assembled sequence. T means 'transmembrane region', S means 'signal peptide'. 4Gene is known (•) to be expressed in fire ant (unpublished microarray data). 5In honey bee, EST evidence exists (•) within 5,000 bp of the aligned region. 6This column shows the annotation of overlapping or nearby (within 5,000 bp) honey bee genes, as well as the nearby presence of genes from non-hymenopteran organisms. Numbers starting with GB are honeybee Official Gene Set numbers. 'Ab initio prediction' indicates that Gnomon, Genscan, or another algorithm was used to predict a gene that was not retained for the bee genome Official Gene Set. 'NH homology' indicates the nearby presence of a gene from non-hymenopteran organisms. 7Based on visual inspection we assigned a confidence level (the more asterisks the better) to each ant-bee putative gene pair (see Materials and methods). 8Apis mellifera unanchored scaffolds such as NW_001254419.1 are regions that have not been mapped to a chromosome. 9Multiple alignment frames for a S. invicta transcript indicate possible frameshifts during sequencing.