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Table 1 Fire ant EST and assembly statistics

From: An annotated cDNA library and microarray for large-scale gene-expression studies in the ant Solenopsis invicta

Total number of sequence reads

28,133

   cDNA clones sequenced from 5' end

22,560

   Extra reads due to re-sequencing

5,573

High-quality sequences after filtering*

21,715

Average EST size after trimming (bp)

522.4

Total number of assembled sequences

11,864

   Number of contigs

4,319

True contigs (from >2 different clones)

3,057

Re-sequencing contigs†

1,262

   Number of singletons

7,545

Number of putatively different fire ant sequences

<11,864

Average size of assembled sequences (bp)

600.5

  1. *High quality sequences are those with greater than 200 bp after trimming of vector and primer sequences and with a phred value higher than 15. In addition, this set excludes artifactual sequences that were manually removed. †Contigs composed of replicate sequences of only one clone