Mode of regulation | Number of genes | Model |
---|
No response | 9,121 | NA |
-N independent | 445 | 1 |
+N independent | 319 | 1 |
-C independent | 1,461 | 2 |
+C independent | 1,104 | 2 |
+C | 331 | 3 |
-C | 157 | 3 |
+CN | 152 | 3 |
-C -CN -N | 103 | 3 |
-CN | 81 | 3 |
+N | 76 | 3 |
-C -N | 71 | 3 |
-N | 49 | 3 |
--C -CN | 40 | 3 |
+C +N | 33 | 3 |
++C +CN | 28 | 3 |
++CN +N | 20 | 3 |
-CN +N | 17 | 3 |
+C -CN | 16 | 3 |
++C +CN +N | 15 | 3 |
-C +CN | 9 | 3 |
-C -CN +N | 5 | 3 |
--CN -N | 2 | 3 |
+C -CN -N | 2 | 3 |
+CN -N | 1 | 3 |
-C +CN +N | 1 | 3 |
Int | 337 | 3 |
+C (+C-N) -N | 60 | 3 (additive) |
-C (-C-N) -N | 136 | 3 (additive) |
+C (+C+N) +N | 172 | 3 (additive) |
-C (-C+N) +N | 98 | 3 (additive) |
- Combinations of letters and plus or minus signs denote the effect of the inputs on regulation of gene expression (for example, +C indicates induction in treatments with carbon). The number of plus or minus signs indicates relative strength of induction (or repression). For model 3, response is observed only for those conditions indicated. For example, +C in model 3 indicates induction in treatments with carbon only and no response for C+N or N treatments. The last four rows of the table contain patterns of additive interactions between C and N. For these patterns of regulation, expression of genes in the C+N treatments was equivalent to adding the expression level in the C-only and the N-only treatments. For a graphical representation of the patterns see Figure S1 (in Additional data file 2). Int, interaction term was found significant by ANOVA analysis but small differences in gene expression between treatments precluded classification by post hoc analysis. This group was not analyzed further.