Skip to main content

Table 1 Functional characterization of non-coding elements significantly conserved only in primates

From: Detection of weakly conserved ancestral mammalian regulatory sequences by primate comparisons

 

In vitro (HepG2)

In vivo (mice)

Primate specific element

DNase I HS

Reporter transfection

Gene transfer

LDLR_PS1

No

No activity

No activity

LDLR_PS2

Yes

Enhancer* (~5.1-fold)

Enhancer (~5.5-fold)

LDLR_PS3

No

No activity

No activity

LDLR_PS4

Yes

Enhancer (~3.7-fold)

Enhancer (~4.2-fold)

SREBF1_PS

No

Silencer (~2.4-fold)

Silencer (~1.8-fold)

CYP7A1_PS

No

No activity

No activity

  1. *In 293T cells. Human elements with primate-specific conservation were tested for their ability to drive reporter gene expression in vitro in HepG2 cells and in vivo in mouse liver. The genomic regions containing primate-conserved elements were also examined for the presence of DNase I hypersensitive sites (DNase I HS) in HepG2 cells. Enhancer or silencer strength is shown as fold increase or decrease relative to the promoter alone in luciferase assays.
Back to article page

Genome Biology

ISSN: 1474-760X

Contact us