Table 2 Experimental conditions and statistics on database searches of 10 slices of pooled urine sample
| Â | Pooled 1 | Pooled 2 | Pooled 3 | Pooled 4 | Pooled 5 | Pooled 6 | Pooled 7 | Pooled 8 | Pooled 9 | Pooled 10 |
|---|---|---|---|---|---|---|---|---|---|---|
Protein separation | Invitrogen NuPAGE 4-12% Bis-Tris 1D Gel | |||||||||
Digestion | In-gel | |||||||||
LC gradient time | 140 min | |||||||||
Identified IT-MS2 spectra by Mascot | 42,578 | 36,288 | 46,328 | 42,664 | 48,938 | 46,529 | 48,101 | 50,654 | 26,607 | 26,817 |
Number of unique peptidesa | 777 | 1133 | 1841 | 1114 | 1591 | 2493 | 2179 | 878 | 1671 | 2006 |
Number of identified proteinsa | 125 | 186 | 290 | 186 | 229 | 302 | 239 | 96 | 206 | 153 |
Total number of unique peptidesa | 9737 | |||||||||
Total number of identified proteinsa | 1055 | |||||||||