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Table 2 Experimental conditions and statistics on database searches of 10 slices of pooled urine sample

From: The human urinary proteome contains more than 1500 proteins, including a large proportion of membrane proteins

  Pooled 1 Pooled 2 Pooled 3 Pooled 4 Pooled 5 Pooled 6 Pooled 7 Pooled 8 Pooled 9 Pooled 10
Protein separation Invitrogen NuPAGE 4-12% Bis-Tris 1D Gel
Digestion In-gel
LC gradient time 140 min
Identified IT-MS2 spectra by Mascot 42,578 36,288 46,328 42,664 48,938 46,529 48,101 50,654 26,607 26,817
Number of unique peptidesa 777 1133 1841 1114 1591 2493 2179 878 1671 2006
Number of identified proteinsa 125 186 290 186 229 302 239 96 206 153
Total number of unique peptidesa 9737
Total number of identified proteinsa 1055
  1. aApplied criteria are described in the Materials and methods section.