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Figure 9 | Genome Biology

Figure 9

From: Statistical methods and software for the analysis of highthroughput reverse genetic assays using flow cytometry readouts

Figure 9

Plate plots show several aspects of the data in a format resembling a microtiter plate. This is useful for detecting spatial effects and to present concisely the data belonging to one experiment. (a) Quantitative values: number of cells in the well. The consistently lower number of cells at the edges of the plate indicate problems during cultivation. (b) Qualitative values: activators (red) and inhibitors (blue) of the process of interest. Wells that did not pass quality requirements are crossed out and wells containing cells treated with controls are indicated by capital letters. Cells in the first four rows of the plate were transfected with amino-terminally tagged expression constructs, and rows five to eight with carboxyl-terminally tagged constructs. (c) Comparison of results from four replicate plates. Each slice contains data from one replicate. Reproducibility between replicates is very high.

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