Figure 11

Separation of positive and negative controls. Top panels: effect sizes of positive and negative controls (y-axis) for individual plates (x-axis). Bottom panels: density plots of the joint effect sizes for controls across all plates. (a) Controls for the apoptosis assay are CIDE3 (positive) and CDK (negative). (b) Controls for the proliferation assay are cyclin A (positive) and YFP (negative). (c) Controls for the MAPK assay are DUSP10 (positive) and YFP (negative). The measured effect sizes for positive and negative controls separate well. CDK, cyclin-dependent kinase; DUSP, dual specificity protein phosphatase; MAPK, mitogen-activated protein kinase; YFP, yellow fluorescent protein.