|
Interaction Gene1 + Gene2
|
Gene1
|
Gene2
|
Gene1 + 2
|
p value
|
|---|
| |
BS
|
ES
|
BS
|
ES
|
BS
|
ES
|
BS
|
ES
|
|---|
|
pab-1 + pab-2
|
15
|
100
|
88
|
100
|
0
|
ns
|
1.9 × 10-04
|
ns
|
|
rpl-25.2 + rpl-25.1
|
6
|
50
|
17
|
63
|
0
|
ns
|
3.6 × 10-04
|
ns
|
|
ptr-2 + ptr-10
|
a
|
53
|
a
|
98
|
a
|
ns
|
a
|
ns
|
|
unc-78 + tag-216
|
85
|
96
|
98
|
97
|
0
|
ns
|
6.4 × 10-15
|
ns
|
|
rab-8 + rab-10
|
87
|
98
|
70
|
96
|
1
|
ns
|
7.3 × 10-05
|
ns
|
|
B0495.2 + ZC504.3
|
84
|
99
|
97
|
99
|
2
|
55
|
6.3 × 10-09
|
2.9 × 10-03
|
|
rpa-2 + C37A2.7
|
67
|
74
|
50
|
81
|
1
|
ns
|
1.9 × 10-07
|
ns
|
|
C28H8.4 + erd-2
|
93
|
95
|
86
|
94
|
10
|
10
|
5.6 × 10-08
|
2.2 × 10-15
|
|
lin-12 + glp-1
|
90
|
88
|
99
|
83
|
16
|
75
|
1.2 × 10-13
|
7.3 × 10-01
|
|
C13G3.3 + W08G11.4
|
73
|
94
|
80
|
97
|
17
|
89
|
1.6 × 10-06
|
3.5 × 10-01
|
|
lin-53 + rba-1
|
74
|
63
|
48
|
5
|
16
|
75
|
1.1 × 10-02
|
7.3 × 10-17
|
|
Y53C12A.4 + R02E12.2
|
84
|
81
|
78
|
87
|
32
|
75
|
1.3 × 10-03
|
6.9 × 10-01
|
|
F37C12.7 + acs-17
|
95
|
100
|
77
|
98
|
44
|
73
|
9.4 × 10-03
|
4.2 × 10-06
|
|
C05G5.4 + F23H11.3
|
96
|
100
|
94
|
98
|
58
|
72
|
5.1 × 10-06
|
1.5 × 10-08
|
|
elt-6 + egl-18
|
100
|
97
|
82
|
88
|
63
|
73
|
4.0 × 10-02
|
6.3 × 10-03
|
|
dsh-1 + dsh-2
|
97
|
98
|
75
|
54
|
58
|
17
|
1.6 × 10-02
|
1.1 × 10-11
|
- C. elegans duplicate gene pairs (Interaction Gene1 + Gene2) displaying synthetic phenotypic effects upon combinatorial RNA interference (RNAi) in the RNAi-hypersensitive strain rrf-3 [19] are listed. Numbers shown are percentages of average wild-type brood size (BS) and embryonic survival (ES) rates for each gene individually (Gene1 or Gene2) as well as for duplicate gene pairs (Gene1 + 2), and are the arithmetic mean of two independent biological repeats. p values were assigned using a Student's t-test. aNote that combinatorial RNAi against the duplicate gene pair ptr-2 + ptr-10 resulted in an increased number of first generation larval growth arrested worms, rather than in reduced brood size; fraction of population which is wild-type (does not arrest at an early larval stage): 70% (ptr-2), 100% (ptr-10), 0% (ptr-2 + ptr-10), p = 7.4 × 10-09. ns, given phenotype could not be scored.
