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Table 3 Genetic interactions of synthetic multivulval genes can be recapitulated by combinatorial RNAi

From: Combinatorial RNA interference in Caenorhabditis elegans reveals that redundancy between gene duplicates can be maintained for more than 80 million years of evolution

SynMuv gene Predicted gene Locus SynMuv pathway Wild-type rrf-3
lin-15B T27C4.4 egr-1 A - -
  ZK678.1 lin-15A A Muv Muv
  K12C11.2 smo-1 A, B ns ns
  W02A11.4 uba-2 A, B Muv Muv
lin-15A K12C11.2 smo-1 A, B ns ns
  W02A11.4 uba-2 A, B - Muv
  C32F10.2 lin-35 B Muv Muv
  C47D12.1 trr-1 B ns ns
  C53A5.3 hda-1/gon-10 B ns ns
  E01A2.4   B - -
  F44B9.6 lin-36 B - Muv
  JC8.6   B ns ns
  K07A1.12 lin-53/rba-2 B ns ns
  M04B2.1 mep-1/gei-2 B - Muv
  R05D3.11 met-2 B - Muv
  R06C7.7 rls-1/lin-61 B Muv Muv
  W01G7.3   B ns ns
  W07B3.2 gei-4 B ns ns
  Y71G12B.9   B - Muv
  Y1O2A5C.18 efl-1 B Muv Muv
  ZK632.13 lin-52 B Muv Muv
  ZK637.7 lin-9 B Muv Muv
  ZK662.4 lin-15B B Muv Muv
  1. Previously studied synthetic multivulval (synMuv) genes were targeted by combinatorial RNA interference (RNAi) in wild-type or RNAi-hypersensitive rrf-3 worms [19]. We show predicted gene name, its corresponding genetic locus name, a definition of the gene as a component of either the synMuv A (A), synMuv B (B), or both (A, B) pathways. All synMuv A genes were targeted by RNAi in combination with a double-stranded (ds)RNA-expressing strain targeting the synMuv B gene lin-15B; corresponding experiments were performed with synMuv B genes and a dsRNA-expressing strain targeting lin-15A. In both cases, worms were scored for the presence of the multivulva (Muv) phenotype. -, absence of Muv phenotype; ns, not scored (RNAi resulted in embryonic lethality or sterility).