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Table 1 Combinatorial RNAi effectively generates additive phenotypes

From: Combinatorial RNA interference in Caenorhabditis elegans reveals that redundancy between gene duplicates can be maintained for more than 80 million years of evolution

Gene1 Gene2 Wild-type rrf-3
   Pheno Gene1 Pheno Gene2 Pheno Gene1 Pheno Gene2
lin-31 - 5% - 35% -
sma-4 - 100% - 100% -
unc-22 - 100% - 100% -
lon-2 - 100% - 100% -
lin-31 sma-4 2% 100% 20% 100%
lin-31 unc-22 2% 100% 26% 100%
lin-31 lon-2 4% 100% 13% 100%
sma-4 unc-22 100% 100% 100% 100%
sma-4 lon-2 100% 0% 100% 0%
unc-22 lon-2 100% 100% 100% 100%
  1. Wild-type and RNA interference (RNAi)-hypersensitive rrf-3 worms, respectively, were fed on selected bacterial strains of the C. elegans RNAi feeding library [2] targeting the genes lin-31, sma-4, unc-22, and lon-2. Independent RNAi phenotypes (Pheno Gene1, Pheno Gene2) were assessed when each gene was targeted individually and also for all possible pair-wise combinations of genes. Percentages represent penetrance of phenotypes.